| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holland, Elle C (16)
|
NITWST |
29.15 |
28.94 |
-0.21 |
| 2 |
Burgard, Sydney R (16)
|
NITWST |
31.68 |
30.19 |
-1.49 |
| 3 |
Kisker, Elizabeth M (16)
|
NITWST |
38.63 |
30.34 |
-8.29 |
| 4 |
Fischer, Mackenzie A (15)
|
NITWST |
30.08 |
30.57 |
0.49 |
| 5 |
Kubiak, Maria T (15)
|
NITWST |
29.99 |
30.63 |
0.64 |
| 6 |
Nicholl, Daisy K (16)
|
NIEAST |
33.04 |
30.95 |
-2.09 |
| 7 |
Clouser, KaRynn F (15)
|
NIBCSR |
30.60 |
31.01 |
0.41 |
| 8 |
Yunke, Julia A (15)
|
NITWST |
30.11 |
31.16 |
1.05 |
| 9 |
Herman, Olivia T (15)
|
NIUN-T |
31.20 |
31.50 |
0.30 |
| 10 |
Raymond, Gabriella M (16)
|
NIHAMB |
46.08 |
46.79 |
0.71 |